Journal: bioRxiv

Article Title: Starve-Feed Cycles Direct Quiescence to Proliferation Transitions in Drosophila Follicle Stem Cells via Transcriptional Regulation

doi: 10.64898/2026.01.22.701111

Figure Lengend Snippet: A-F) Germaria isolated from transgenic fly lines with Gal4 inserted into genes known to be expressed and functional in the germarium. Probes complementary to Gal4 (green) and Fas3 (red) were used for RNA in situ hybridization using the RNAScope approach. Nuclei are labeled (Draq5, blue). Scale bars are indicated for each image.

Article Snippet: Draq5 was used to label nuclei (1:1000, Miltenyi Biotec Cat# 130-117-344).

Techniques: Isolation, Transgenic Assay, Functional Assay, RNA In Situ Hybridization, RNAscope, Labeling

Journal: bioRxiv

Article Title: Starve-Feed Cycles Direct Quiescence to Proliferation Transitions in Drosophila Follicle Stem Cells via Transcriptional Regulation

doi: 10.64898/2026.01.22.701111

Figure Lengend Snippet: RNAScope of transgenic lines bearing insertion of Gal4 in Q->P regulatory candidates, using probes against Gal4 (green) and Fas3 (red). Nuclei are labeled (Draq5, blue). Candidates with no detectable Gal4 (top) or broad, non-specific expression (middle, bottom) are shown. Scale bars are indicated for each image.

Article Snippet: Draq5 was used to label nuclei (1:1000, Miltenyi Biotec Cat# 130-117-344).

Techniques: RNAscope, Transgenic Assay, Labeling, Expressing

Journal: bioRxiv

Article Title: Starve-Feed Cycles Direct Quiescence to Proliferation Transitions in Drosophila Follicle Stem Cells via Transcriptional Regulation

doi: 10.64898/2026.01.22.701111

Figure Lengend Snippet: RNAScope of transgenic lines bearing insertion of Gal4 in Q->P regulatory candidates, using probes against Gal4 (green) and Fas3 (red). Nuclei are labeled (Draq5, blue). Candidates with Gal4 in cells other than FSCs (A), or enriched in FSCs (B) are shown. The FSC region is indicated by a dotted oval in B. Scale bars are indicated for each image.

Article Snippet: Draq5 was used to label nuclei (1:1000, Miltenyi Biotec Cat# 130-117-344).

Techniques: RNAscope, Transgenic Assay, Labeling

Journal: bioRxiv

Article Title: Starve-Feed Cycles Direct Quiescence to Proliferation Transitions in Drosophila Follicle Stem Cells via Transcriptional Regulation

doi: 10.64898/2026.01.22.701111

Figure Lengend Snippet: A-E) RNAScope of germaria bearing Gal4 expression in the indicated genes. Probes against Gal4 (green) and Fas3 (red) indicate gene expression in nutrient-restricted (“starved”) or 6-hours fed germaria. Nuclei are labeled (Draq5, blue). The FSC niche is outlined by a dashed oval. Scale bars are indicated for each image. A) Feeding-dependence of previously characterized genes. B) Candidates in the Aq2 cluster, which are enriched in the TU-tagged fraction and increase by 3 hours after feeding. C) Candidates in the Aq1 cluster, which are enriched in the TU-tagged fraction and increase in both the Input and the streptavidin-precipitated fraction by 3 hours after feeding. D) Candidates in the bq1 cluster, which are enriched in the TU-tagged fraction and increase in both the Input and the streptavidin-precipitated fraction by 6 hours after feeding. E) nrv1-Gal4 germarium, a non-feeding-dependent example that shares function with nrv2 , a feeding-dependent gene.

Article Snippet: Draq5 was used to label nuclei (1:1000, Miltenyi Biotec Cat# 130-117-344).

Techniques: RNAscope, Expressing, Gene Expression, Labeling

Journal: Development (Cambridge, England)

Article Title: Single-cell transcriptomic profiling of the whole colony of Botrylloides diegensis : insights into tissue specialization and blastogenesis

doi: 10.1242/dev.204265

Figure Lengend Snippet: Overview of the experimental pipeline and clustering results. (A) Single cells were prepared using the acetic methanol (ACME) maceration method . Cells were stained with DRAQ5 and sorted by FACS. Cells were captured in droplets using a 10x Chromium system. Single-cell libraries were prepared and sequenced. After mapping the transcripts to the genome, clustering analysis was performed to identify the cell types. (B) UMAP clustering analysis revealed 29 single-cell clusters. Each cluster was color-coded. (C) Heatmap of the top five marker genes in each cluster.

Article Snippet: ACME-fixed cells were stained with the DNA dye DRAQ5 to sort intact single cells from cellular debris and aggregates (eBioscience 0.66 μl/ml of 5 mM stock).

Techniques: Staining, Marker